X-ray diffraction
2.42Å resolution

Crystal structure of TrmD, a tRNA-(N1G37) methyltransferase, from Mycobacterium abscessus in complex with inhibitor


Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
tRNA (guanine-N(1)-)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 244 amino acids
Theoretical weight: 26.43 KDa
Source organism: Mycobacteroides abscessus
Expression system: Escherichia coli
  • Canonical: B1MDI3 (Residues: 1-242; Coverage: 100%)
Gene names: MAB_3226c, trmD
Sequence domains: tRNA (Guanine-1)-methyltransferase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 75.679Å b: 77.179Å c: 87.556Å
α: 90° β: 90° γ: 90°
R R work R free
0.185 0.182 0.246
Expression system: Escherichia coli