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Influenza A virus Polymerase Heterotrimer A/duck/Fujian/01/2002(H5N1)

X-ray diffraction
3.634Å resolution
PDB entry 6qpf coloured by chain, front view.
PDB entry 6qpf coloured by chain, side view.
PDB entry 6qpf coloured by chain, top view.
Source organism: Influenza A virus (A/duck/Fujian/01/2002(H5N1))
Assembly composition:
hetero trimer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero trimer
Accessible surface area: 95614.67 Å2
Buried surface area: 33271.36 Å2
Dissociation area: 9,383.71 Å2
Dissociation energy (ΔGdiss): 115.41 kcal/mol
Dissociation entropy (TΔSdiss): 18.13 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-112588
Assembly 2
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Multimeric state: hetero trimer
Accessible surface area: 97437.82 Å2
Buried surface area: 32479.3 Å2
Dissociation area: 9,046.42 Å2
Dissociation energy (ΔGdiss): 118.48 kcal/mol
Dissociation entropy (TΔSdiss): 18.06 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-112588
Assembly 3
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Multimeric state: hetero trimer
Accessible surface area: 95805.85 Å2
Buried surface area: 34123.19 Å2
Dissociation area: 9,914.03 Å2
Dissociation energy (ΔGdiss): 124.23 kcal/mol
Dissociation entropy (TΔSdiss): 18.24 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-112588
Assembly 4
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Multimeric state: hetero trimer
Accessible surface area: 94096.4 Å2
Buried surface area: 32963.75 Å2
Dissociation area: 9,221.67 Å2
Dissociation energy (ΔGdiss): 116.52 kcal/mol
Dissociation entropy (TΔSdiss): 18.09 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-112588

Macromolecules

Polymerase acidic protein
Chains: A, D, G, J
Length: 716 amino acids
Theoretical weight: 82.71 KDa
Source organism: Influenza A virus (A/duck/Fujian/01/2002(H5N1))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: D3YNB8 (Residues: 1-716; Coverage: 100%)
Gene name: PA
Pfam: Influenza RNA-dependent RNA polymerase subunit PA
InterPro:
Polymerase acidic protein in PDB entry 6qpf, assembly 1, front view.
Polymerase acidic protein in PDB entry 6qpf, assembly 1, side view.
Polymerase acidic protein in PDB entry 6qpf, assembly 1, top view.
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RNA-directed RNA polymerase catalytic subunit
Chains: B, E, H, K
Length: 757 amino acids
Theoretical weight: 86.55 KDa
Source organism: Influenza A virus (A/duck/Fujian/01/2002(H5N1))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: D3YNB6 (Residues: 1-757; Coverage: 100%)
Gene name: PB1
Pfam: Influenza RNA-dependent RNA polymerase subunit PB1
InterPro:
RNA-directed RNA polymerase catalytic subunit in PDB entry 6qpf, assembly 1, front view.
RNA-directed RNA polymerase catalytic subunit in PDB entry 6qpf, assembly 1, side view.
RNA-directed RNA polymerase catalytic subunit in PDB entry 6qpf, assembly 1, top view.
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Polymerase basic protein 2
Chains: C, F, I, L
Length: 765 amino acids
Theoretical weight: 86.82 KDa
Source organism: Influenza A virus (A/duck/Fujian/01/2002(H5N1))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: D3YNB5 (Residues: 1-759; Coverage: 100%)
Gene name: PB2
Pfam:
InterPro:
Polymerase basic protein 2 in PDB entry 6qpf, assembly 1, front view.
Polymerase basic protein 2 in PDB entry 6qpf, assembly 1, side view.
Polymerase basic protein 2 in PDB entry 6qpf, assembly 1, top view.
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