› 6qni

X-ray diffraction
1.85Å resolution

Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Xylose Isomerase 1.0 s timepoint

Released:
DOI: 10.2210/pdb6qni/pdb
PDB entry 6qni coloured by chain, front view.
PDB entry 6qni coloured by chain, side view.
PDB entry 6qni coloured by chain, top view.
Source organism: Streptomyces rubiginosus
Primary publication:
Liquid application method for time-resolved analyses by serial synchrotron crystallography.
Mehrabi P, Schulz EC, Agthe M, Horrell S, Bourenkov G, von Stetten D, Leimkohl JP, Schikora H, Schneider TR, Pearson AR, Tellkamp F, Miller RJD
Nat Methods 16 979-982 (2019)
PMID: 31527838

Function and Biology Details

Reaction catalysed: 
Alpha-D-xylopyranose = alpha-D-xylufuranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150202 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xylose isomerase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.28 KDa
Source organism: Streptomyces rubiginosus
Expression system: Streptomyces rubiginosus
UniProt:
  • Canonical: P24300 (Residues: 1-388; Coverage: 100%)
Gene name: xylA
Sequence domains: Xylose isomerase-like TIM barrel

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand GLC 1 x GLC
Ligand CO 1 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: I222
Unit cell:
a: 94.62Å b: 99.38Å c: 87.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.196
Expression system: Streptomyces rubiginosus

Quick links

Citations

7 review citations

Serial synchrotron and XFEL crystallography for studies of metalloprotein catalysis.
Hough et al. (2021)
6 more

1 mention without citation

A simple vapor-diffusion method enables protein crystallization inside the HARE serial crystallography chip.
Norton-Baker et al. (2021)