› 6qm4

Electron Microscopy
3.8Å resolution

Cryo-EM structure of calcium-free nhTMEM16 lipid scramblase in nanodisc

Released:
DOI: 10.2210/pdb6qm4/pdb
PDB entry 6qm4 coloured by chain, front view.
PDB entry 6qm4 coloured by chain, side view.
PDB entry 6qm4 coloured by chain, top view.
Source organism: Fusarium vanettenii 77-13-4
Primary publication:
Stepwise activation mechanism of the scramblase nhTMEM16 revealed by cryo-EM.
Kalienkova V, Clerico Mosina V, Bryner L, Oostergetel GT, Dutzler R, Paulino C
Elife 8 (2019)
PMID: 30785398
Related structures: EMD-4587

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111787 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plasma membrane channel protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 735 amino acids
Theoretical weight: 83.2 KDa
Source organism: Fusarium vanettenii 77-13-4
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: C7Z7K1 (Residues: 1-735; Coverage: 100%)
Gene name: NECHADRAFT_66456
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-4587
Expression system: Saccharomyces cerevisiae

Quick links

Citations

11 review citations

Flagging fusion: Phosphatidylserine signaling in cell-cell fusion.
Whitlock et al. (2021)
10 more

6 mentions without citation

Gating and Regulatory Mechanisms of TMEM16 Ion Channels and Scramblases.
Le et al. (2021)
5 more