› 6qm2

X-ray diffraction
2.8Å resolution

NlaIV restriction endonuclease

Released:
DOI: 10.2210/pdb6qm2/pdb
PDB entry 6qm2 coloured by chain, front view.
PDB entry 6qm2 coloured by chain, side view.
PDB entry 6qm2 coloured by chain, top view.
Source organism: Neisseria lactamica
Primary publication:
Crystal Structure and Directed Evolution of Specificity of NlaIV Restriction Endonuclease.
Czapinska H, Siwek W, Szczepanowski RH, Bujnicki JM, Bochtler M, Skowronek KJ
J Mol Biol 431 2082-2094 (2019)
PMID: 30995450

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156143 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II restriction enzyme NlaIV Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 29.15 KDa
Source organism: Neisseria lactamica
Expression system: Escherichia coli
UniProt:
  • Canonical: P50183 (Residues: 1-243; Coverage: 100%)
Gene name: nlaIVR
Sequence domains: NgoBV restriction endonuclease

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P6222
Unit cell:
a: 110.38Å b: 110.38Å c: 209.006Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.191 0.221
Expression system: Escherichia coli

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