› 6qie

X-ray diffraction
2.7Å resolution

Crystal structure of DEAH-box ATPase Prp43-S387G

Released:
DOI: 10.2210/pdb6qie/pdb
PDB entry 6qie coloured by chain, front view.
PDB entry 6qie coloured by chain, side view.
PDB entry 6qie coloured by chain, top view.
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Primary publication:
Structural basis for RNA translocation by DEAH-box ATPases.
Hamann F, Enders M, Ficner R
Nucleic Acids Res 47 4349-4362 (2019)
PMID: 30828714

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123894 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA helicase Chain: A
Molecule details ›
Chain: A
Length: 705 amino acids
Theoretical weight: 80.42 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0RY84 (Residues: 61-764; Coverage: 92%)
Gene name: CTHT_0005780
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand MPD 9 x MPD
Ligand ADP 1 x ADP
Ligand CL 1 x CL
Ligand SO4 5 x SO4
Ligand MG 1 x MG
Ligand BEF 1 x BEF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P212121
Unit cell:
a: 88.64Å b: 102.98Å c: 118.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.229 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

DEAH-Box RNA Helicases in Pre-mRNA Splicing.
De Bortoli et al. (2021)
3 more

1 mention without citation

Structural basis for RNA translocation by DEAH-box ATPases.
Hamann et al. (2019)