6qdt

X-ray diffraction
1.7Å resolution

Crystal structure of 14-3-3sigma in complex with a RapGef2 pT740 phosphopeptide

Released:
Source organism: Homo sapiens
Entry authors: Andrei SA, Kaplan A, Fournier AE, Ottman C

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Rap guanine nucleotide exchange factor 2 Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.33 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9Y4G8 (Residues: 747-757; Coverage: 1%)
Gene names: KIAA0313, NRAPGEP, PDZGEF1, RAPGEF2

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-003
Spacegroup: C2221
Unit cell:
a: 82.657Å b: 112.411Å c: 62.749Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.192
Expression systems:
  • Escherichia coli
  • Not provided