› 6qdt

X-ray diffraction
1.7Å resolution

Crystal structure of 14-3-3sigma in complex with a RapGef2 pT740 phosphopeptide

Released:
DOI: 10.2210/pdb6qdt/pdb
PDB entry 6qdt coloured by chain, front view.
PDB entry 6qdt coloured by chain, side view.
PDB entry 6qdt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Polypharmacological Perturbation of the 14-3-3 Adaptor Protein Interactome Stimulates Neurite Outgrowth.
Kaplan A, Andrei SA, van Regteren Altena A, Simas T, Banerjee SL, Kato N, Bisson N, Higuchi Y, Ottmann C, Fournier AE
Cell Chem Biol 27 657-667.e6 (2020)
PMID: 32220335

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152173 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Rap guanine nucleotide exchange factor 2 Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.33 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9Y4G8 (Residues: 747-757; Coverage: 1%)
Gene names: KIAA0313, NRAPGEP, PDZGEF1, RAPGEF2

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand CL 1 x CL
Ligand NA 5 x NA
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-003
Spacegroup: C2221
Unit cell:
a: 82.657Å b: 112.411Å c: 62.749Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.192
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

The Surprising Story of Fusicoccin: A Wilt-Inducing Phytotoxin, a Tool in Plant Physiology and a 14-3-3-Targeted Drug.
Marra et al. (2021)
4 more