› 6qd4

Electron Microscopy
5.6Å resolution

MloK1 model from single particle analysis of 2D crystals, class 8 (intermediate conformation)

Released:
DOI: 10.2210/pdb6qd4/pdb
PDB entry 6qd4 coloured by chain, front view.
PDB entry 6qd4 coloured by chain, side view.
PDB entry 6qd4 coloured by chain, top view.
Source organism: Mesorhizobium japonicum MAFF 303099
Primary publication:
Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM.
Righetto RD, Biyani N, Kowal J, Chami M, Stahlberg H
Nat Commun 10 1722 (2019)
PMID: 30979902
Related structures: EMD-4519

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189151 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic nucleotide-gated potassium channel mll3241 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 355 amino acids
Theoretical weight: 37.77 KDa
Source organism: Mesorhizobium japonicum MAFF 303099
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q98GN8 (Residues: 1-355; Coverage: 100%)
Gene name: mll3241
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.6Å
Relevant EMDB volumes: EMD-4519
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Macromolecular crystallography using microcrystal electron diffraction.
Clabbers et al. (2021)
3 more