6qbx

Electron Microscopy
4.2Å resolution

Ovine respiratory supercomplex I+III2 closed class.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 65-mer (preferred)
Entry contents:
54 distinct polypeptide molecules
Macromolecules (54 distinct):
Cytochrome b-c1 complex subunit 1, mitochondrial Chains: a1, a3
Molecule details ›
Chains: a1, a3
Length: 446 amino acids
Theoretical weight: 49.39 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5Q5G6 (Residues: 35-483; Coverage: 92%)
Gene name: LOC101110095
Sequence domains:
Cytochrome b-c1 complex subunit 2, mitochondrial Chains: a2, a4
Molecule details ›
Chains: a2, a4
Length: 439 amino acids
Theoretical weight: 46.66 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5Q0F9 (Residues: 15-453; Coverage: 97%)
Gene name: LOC101102527
Sequence domains:
Cytochrome b Chains: b1, b2
Molecule details ›
Chains: b1, b2
Length: 379 amino acids
Theoretical weight: 42.88 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: P24959 (Residues: 1-379; Coverage: 100%)
Gene names: COB, CYTB, MT-CYB, MTCYB
Sequence domains:
Cytochrome c domain-containing protein Chains: c1, c2
Molecule details ›
Chains: c1, c2
Length: 240 amino acids
Theoretical weight: 27.32 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5Q0A9 (Residues: 85-324; Coverage: 74%)
Gene name: LOC101113599
Sequence domains: Cytochrome C1 family
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chains: f1, f2
Molecule details ›
Chains: f1, f2
Length: 196 amino acids
Theoretical weight: 21.65 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5P2X9 (Residues: 79-274; Coverage: 72%)
Gene name: LOC101113001
Sequence domains:
Cytochrome b-c1 complex subunit 7 Chains: d1, d2
Molecule details ›
Chains: d1, d2
Length: 110 amino acids
Theoretical weight: 13.43 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5P642 (Residues: 1-109; Coverage: 100%)
Sequence domains: Ubiquinol-cytochrome C reductase complex 14kD subunit
Complex III subunit 8 Chains: q1, q2
Molecule details ›
Chains: q1, q2
Length: 81 amino acids
Theoretical weight: 9.61 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PUP9 (Residues: 2-82; Coverage: 99%)
Gene name: LOC101121420
Sequence domains: UcrQ family
Cytochrome b-c1 complex subunit 6 Chains: h1, h2
Molecule details ›
Chains: h1, h2
Length: 78 amino acids
Theoretical weight: 9.22 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: B9VH04 (Residues: 14-91; Coverage: 86%)
Gene name: UQCRH
Sequence domains: Ubiquinol-cytochrome C reductase hinge protein
UQCRFS1N Chains: x1, x2
Molecule details ›
Chains: x1, x2
Length: 78 amino acids
Theoretical weight: 6.66 KDa
Source organism: Ovis aries
Complex III subunit 9 Chains: i1, i2
Molecule details ›
Chains: i1, i2
Length: 63 amino acids
Theoretical weight: 7.31 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5P6B2 (Residues: 2-64; Coverage: 98%)
Gene name: LOC101108778
Sequence domains: Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial Chain: V1
Molecule details ›
Chain: V1
Length: 445 amino acids
Theoretical weight: 48.68 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PUX0 (Residues: 20-464; Coverage: 96%)
Gene name: NDUFV1
Sequence domains:
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial Chain: V2
Molecule details ›
Chain: V2
Length: 217 amino acids
Theoretical weight: 23.84 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5NRY1 (Residues: 30-246; Coverage: 88%)
Gene name: NDUFV2
Sequence domains: Thioredoxin-like [2Fe-2S] ferredoxin
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial Chain: S1
Molecule details ›
Chain: S1
Length: 704 amino acids
Theoretical weight: 77.03 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QB34 (Residues: 24-727; Coverage: 97%)
Gene name: NDUFS1
Sequence domains:
NDUFS2 Chain: S2
Molecule details ›
Chain: S2
Length: 430 amino acids
Theoretical weight: 49.19 KDa
Source organism: Ovis aries
Complex I-30kD Chain: S3
Molecule details ›
Chain: S3
Length: 228 amino acids
Theoretical weight: 26.44 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PB27 (Residues: 39-266; Coverage: 86%)
Gene name: NDUFS3
Sequence domains: Respiratory-chain NADH dehydrogenase, 30 Kd subunit
NDUFS7 Chain: S7
Molecule details ›
Chain: S7
Length: 179 amino acids
Theoretical weight: 20.1 KDa
Source organism: Ovis aries
NDUFS8 Chain: S8
Molecule details ›
Chain: S8
Length: 176 amino acids
Theoretical weight: 20.22 KDa
Source organism: Ovis aries
NDUFV3 Chain: V3
Molecule details ›
Chain: V3
Length: 75 amino acids
Theoretical weight: 8.42 KDa
Source organism: Ovis aries
NDUFS6 Chain: S6
Molecule details ›
Chain: S6
Length: 96 amino acids
Theoretical weight: 10.65 KDa
Source organism: Ovis aries
NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial Chain: S4
Molecule details ›
Chain: S4
Length: 133 amino acids
Theoretical weight: 15.38 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PE07 (Residues: 38-170; Coverage: 78%)
Gene name: NDUFS4
Sequence domains: ETC complex I subunit conserved region
Epimerase domain-containing protein Chain: A9
Molecule details ›
Chain: A9
Length: 338 amino acids
Theoretical weight: 38.66 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PI58 (Residues: 36-373; Coverage: 89%)
Gene name: NDUFA9
Sequence domains: NAD dependent epimerase/dehydratase family
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 Chain: A2
Molecule details ›
Chain: A2
Length: 98 amino acids
Theoretical weight: 10.97 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QAH8 (Residues: 2-99; Coverage: 99%)
Gene name: NDUFA2
Sequence domains: Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain
Complex I subunit B13 Chain: A5
Molecule details ›
Chain: A5
Length: 115 amino acids
Theoretical weight: 13.16 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PNX7 (Residues: 2-116; Coverage: 99%)
Gene name: NDUFA5
Sequence domains: ETC complex I subunit conserved region
Complex I-B14 Chain: A6
Molecule details ›
Chain: A6
Length: 127 amino acids
Theoretical weight: 14.92 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QC06 (Residues: 14-140; Coverage: 91%)
Gene name: NDUFA6
Sequence domains: Complex 1 protein (LYR family)
Complex I-B14.5a Chain: A7
Molecule details ›
Chain: A7
Length: 112 amino acids
Theoretical weight: 12.4 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5P0I2 (Residues: 3-114; Coverage: 98%)
Gene name: NDUFA7
Sequence domains: NADH:ubiquinone oxidoreductase subunit B14.5a (Complex I-B14.5a)
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 Chain: AL
Molecule details ›
Chain: AL
Length: 145 amino acids
Theoretical weight: 17.12 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: B9VGZ9 (Residues: 1-145; Coverage: 100%)
Sequence domains: NADH ubiquinone oxidoreductase subunit NDUFA12
Acyl carrier protein Chains: AA, AB
Molecule details ›
Chains: AA, AB
Length: 88 amino acids
Theoretical weight: 10.12 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5NQT7 (Residues: 70-157; Coverage: 56%)
Sequence domains: Phosphopantetheine attachment site
NDUFA13 Chain: AM
Molecule details ›
Chain: AM
Length: 143 amino acids
Theoretical weight: 16.64 KDa
Source organism: Ovis aries
NADH-ubiquinone oxidoreductase chain 3 Chain: D3
Molecule details ›
Chain: D3
Length: 115 amino acids
Theoretical weight: 13.11 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78753 (Residues: 1-115; Coverage: 100%)
Gene names: MT-ND3, MTND3, NADH3, ND3
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
NADH-ubiquinone oxidoreductase chain 1 Chain: D1
Molecule details ›
Chain: D1
Length: 318 amino acids
Theoretical weight: 35.88 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78747 (Residues: 1-318; Coverage: 100%)
Gene names: MT-ND1, MTND1, NADH1, ND1
Sequence domains: NADH dehydrogenase
NADH-ubiquinone oxidoreductase chain 6 Chain: D6
Molecule details ›
Chain: D6
Length: 175 amino acids
Theoretical weight: 19.13 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78757 (Residues: 1-175; Coverage: 100%)
Gene names: MT-ND6, MTND6, NADH6, ND6
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 6
NADH-ubiquinone oxidoreductase chain 4L Chain: 4L
Molecule details ›
Chain: 4L
Length: 98 amino acids
Theoretical weight: 10.84 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78754 (Residues: 1-98; Coverage: 100%)
Gene names: MT-ND4L, MTND4L, NADH4L, ND4L
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 4L
NADH-ubiquinone oxidoreductase chain 5 Chain: D5
Molecule details ›
Chain: D5
Length: 606 amino acids
Theoretical weight: 68.41 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78756 (Residues: 1-606; Coverage: 100%)
Gene names: MT-ND5, MTND5, NADH5, ND5
Sequence domains:
NADH-ubiquinone oxidoreductase chain 4 Chain: D4
Molecule details ›
Chain: D4
Length: 459 amino acids
Theoretical weight: 52.06 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78755 (Residues: 1-459; Coverage: 100%)
Gene names: MT-ND4, MTND4, NADH4, ND4
Sequence domains:
NADH-ubiquinone oxidoreductase chain 2 Chain: D2
Molecule details ›
Chain: D2
Length: 347 amino acids
Theoretical weight: 39.15 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78748 (Residues: 1-347; Coverage: 100%)
Gene names: MT-ND2, MTND2, NADH2, ND2
Sequence domains:
Complex I-B14.7 Chain: AK
Molecule details ›
Chain: AK
Length: 140 amino acids
Theoretical weight: 14.61 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PAR2 (Residues: 2-141; Coverage: 99%)
Gene name: NDUFA11
Sequence domains: Tim17/Tim22/Tim23/Pmp24 family
Complex I-SGDH Chain: B5
Molecule details ›
Chain: B5
Length: 143 amino acids
Theoretical weight: 16.71 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QHN8 (Residues: 47-189; Coverage: 76%)
Gene name: NDUFB5
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 Chain: A8
Molecule details ›
Chain: A8
Length: 171 amino acids
Theoretical weight: 20.01 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PYA5 (Residues: 2-172; Coverage: 99%)
Gene name: NDUFA8
Sequence domains: CHCH domain
NDUFB10 Chain: BJ
Molecule details ›
Chain: BJ
Length: 175 amino acids
Theoretical weight: 20.88 KDa
Source organism: Ovis aries
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial Chain: AJ
Molecule details ›
Chain: AJ
Length: 320 amino acids
Theoretical weight: 36.8 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QBF5 (Residues: 36-355; Coverage: 90%)
Gene name: NDUFA10
Sequence domains: Deoxynucleoside kinase
Complex I-15 kDa Chain: S5
Molecule details ›
Chain: S5
Length: 105 amino acids
Theoretical weight: 12.47 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QFF9 (Residues: 2-106; Coverage: 99%)
Gene name: NDUFS5
Complex I-B9 Chain: A3
Molecule details ›
Chain: A3
Length: 83 amino acids
Theoretical weight: 9.19 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5NYM7 (Residues: 2-84; Coverage: 99%)
Gene name: NDUFA3
Sequence domains: NADH dehydrogenase 1 alpha subcomplex subunit 3
Complex I-B12 Chain: B3
Molecule details ›
Chain: B3
Length: 97 amino acids
Theoretical weight: 11.02 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5Q5T4 (Residues: 2-98; Coverage: 99%)
Gene name: LOC114108659
Sequence domains: NADH-ubiquinone oxidoreductase B12 subunit family
NADH dehydrogenase [ubiquinone] 1 subunit C2 Chain: C2
Molecule details ›
Chain: C2
Length: 120 amino acids
Theoretical weight: 14.23 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5P9Q8 (Residues: 3-122; Coverage: 98%)
Gene name: NDUFC2
Sequence domains: NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2)
Complex I-B15 Chain: B4
Molecule details ›
Chain: B4
Length: 128 amino acids
Theoretical weight: 14.98 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QF73 (Residues: 3-130; Coverage: 99%)
Gene name: NDUFB4
Sequence domains: NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4)
Complex I-B17 Chain: B6
Molecule details ›
Chain: B6
Length: 127 amino acids
Theoretical weight: 15.43 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PZE3 (Residues: 2-128; Coverage: 99%)
Gene name: NDUFB6
Complex I-B18 Chain: B7
Molecule details ›
Chain: B7
Length: 119 amino acids
Theoretical weight: 14.65 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5P5V3 (Residues: 2-120; Coverage: 87%)
Gene name: NDUFB7
Sequence domains: NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7)
Complex I-B22 Chain: B9
Molecule details ›
Chain: B9
Length: 178 amino acids
Theoretical weight: 21.65 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PGA3 (Residues: 2-179; Coverage: 99%)
Gene name: NDUFB9
Sequence domains: Complex 1 protein (LYR family)
Complex I-AGGG Chain: B2
Molecule details ›
Chain: B2
Length: 72 amino acids
Theoretical weight: 8.5 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PVD7 (Residues: 37-108; Coverage: 67%)
Gene name: NDUFB2
Sequence domains: NADH dehydrogenase 1 beta subcomplex subunit 2
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial Chain: B8
Molecule details ›
Chain: B8
Length: 158 amino acids
Theoretical weight: 18.82 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5Q1B0 (Residues: 29-186; Coverage: 85%)
Gene name: NDUFB8
Complex I-ESSS Chain: BK
Molecule details ›
Chain: BK
Length: 125 amino acids
Theoretical weight: 14.46 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PWF1 (Residues: 30-154; Coverage: 91%)
Gene name: NDUFB11
NDUFC1 Chain: C1
Molecule details ›
Chain: C1
Length: 49 amino acids
Theoretical weight: 5.81 KDa
Source organism: Ovis aries
Complex I-MNLL Chain: B1
Molecule details ›
Chain: B1
Length: 57 amino acids
Theoretical weight: 6.93 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QG39 (Residues: 2-58; Coverage: 98%)
NDUFA1 Chain: A1
Molecule details ›
Chain: A1
Length: 70 amino acids
Theoretical weight: 8.21 KDa
Source organism: Ovis aries

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-4493