› 6q6r

X-ray diffraction
1.5Å resolution

Recognition of different base tetrads by RHAU: X-ray crystal structure of G4 recognition motif bound to the 3-end tetrad of a DNA G-quadruplex

Released:
DOI: 10.2210/pdb6q6r/pdb
PDB entry 6q6r coloured by chain, front view.
PDB entry 6q6r coloured by chain, side view.
PDB entry 6q6r coloured by chain, top view.
Source organisms:
Primary publication:
Recognition of different base tetrads by RHAU (DHX36): X-ray crystal structure of the G4 recognition motif bound to the 3'-end tetrad of a DNA G-quadruplex.
Heddi B, Cheong VV, Schmitt E, Mechulam Y, Phan AT
J Struct Biol 209 107399 (2020)
PMID: 31586599

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120484 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
ATP-dependent DNA/RNA helicase DHX36 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 29 amino acids
Theoretical weight: 3.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H2U1 (Residues: 53-81; Coverage: 3%)
Gene names: DDX36, DHX36, KIAA1488, MLEL1, RHAU
Parallel stranded DNA G-quadruplex Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 16 nucleotides
Theoretical weight: 5.12 KDa
Source organism: unidentified
Expression system: synthetic construct

Ligands and Environments

1 bound ligand:
Ligand K 10 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 56.39Å b: 42.25Å c: 61.404Å
α: 90° β: 99.3° γ: 90°
R-values:
R R work R free
0.188 0.185 0.245
Expression systems:
  • Escherichia coli
  • synthetic construct

Quick links

Citations

3 review citations

G-quadruplex DNA: a novel target for drug design.
Teng et al. (2021)
2 more

3 mentions without citation

G4-quadruplex-binding proteins: review and insights into selectivity.
Meier-Stephenson V. (2022)
2 more