6q2n

Electron Microscopy
4.4Å resolution

Cryo-EM structure of RET/GFRa1/GDNF extracellular complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Glial cell line-derived neurotrophic factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 134 amino acids
Theoretical weight: 15.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P39905 (Residues: 78-211; Coverage: 70%)
Gene name: GDNF
Sequence domains: Transforming growth factor beta like domain
GDNF family receptor alpha-1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 412 amino acids
Theoretical weight: 46.36 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P56159 (Residues: 25-426; Coverage: 91%)
Gene names: GDNFRA, GFRA1, RETL1, TRNR1
Sequence domains: GDNF/GAS1 domain
Extracellular cell-membrane anchored RET cadherin 120 kDa fragment Chains: E, F
Molecule details ›
Chains: E, F
Length: 617 amino acids
Theoretical weight: 69.1 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P07949 (Residues: 29-635; Coverage: 56%)
Gene names: CDHF12, CDHR16, PTC, RET, RET51
Sequence domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-20575
Expression systems:
  • Escherichia coli
  • Homo sapiens