6q2j

Electron Microscopy
4.1Å resolution

Cryo-EM structure of extracellular dimeric complex of RET/GFRAL/GDF15

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Growth/differentiation factor 15 Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 14.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99988 (Residues: 197-308; Coverage: 40%)
Gene names: GDF15, MIC1, PDF, PLAB, PTGFB
Sequence domains: Transforming growth factor beta like domain
GDNF family receptor alpha-like Chains: C, D
Molecule details ›
Chains: C, D
Length: 343 amino acids
Theoretical weight: 39.12 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6UXV0 (Residues: 20-352; Coverage: 89%)
Gene names: C6orf144, GFRAL, UNQ9356/PRO34128
Sequence domains: GDNF/GAS1 domain
Extracellular cell-membrane anchored RET cadherin 120 kDa fragment Chains: E, F
Molecule details ›
Chains: E, F
Length: 617 amino acids
Theoretical weight: 69.1 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P07949 (Residues: 29-635; Coverage: 56%)
Gene names: CDHF12, CDHR16, PTC, RET, RET51
Sequence domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-20572
Expression systems:
  • Escherichia coli
  • Homo sapiens