› 6q1l

X-ray diffraction
1.6Å resolution

Crystal structure of oxidized iodotyrosine deiodinase (IYD) bound to FMN and 3-iodo-L-tyrosine

Released:
DOI: 10.2210/pdb6q1l/pdb
PDB entry 6q1l coloured by chain, front view.
PDB entry 6q1l coloured by chain, side view.
PDB entry 6q1l coloured by chain, top view.
Source organism: Thermotoga neapolitana DSM 4359
Primary publication:
The minimal structure for iodotyrosine deiodinase function is defined by an outlier protein from the thermophilic bacterium Thermotoga neapolitana.
Sun Z, Xu B, Spisak S, Kavran JM, Rokita SE
J Biol Chem 297 101385 (2021)
PMID: 34748729

Function and Biology Details

Reaction catalysed: 
(1a) L-tyrosine + NADP(+) + iodide = 3-iodo-L-tyrosine + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110846 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iodotyrosine deiodinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 22.89 KDa
Source organism: Thermotoga neapolitana DSM 4359
Expression system: Escherichia coli
UniProt:
  • Canonical: B9K712 (Residues: 1-186; Coverage: 100%)
Gene names: CTN_0569, IYD
Sequence domains: Nitroreductase family

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
2 bound ligands:
Ligand IYR 2 x IYR
Ligand CL 6 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 42.861Å b: 81.232Å c: 103.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.203
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Sequence Conservation Does Not Always Signify a Functional Imperative as Observed in the Nitroreductase Superfamily.
Musila et al. (2022)
1 more

2 mentions without citation

The minimal structure for iodotyrosine deiodinase function is defined by an outlier protein from the thermophilic bacterium Thermotoga neapolitana.
Sun et al. (2021)
1 more