6q0t

Electron Microscopy
5.7Å resolution

Structure of a MAPK pathway complex

Released:
Source organisms:
Entry authors: Park E, Rawson S, Jeon H, Eck MJ
Related structures: EMD-20552

Function and Biology Details

Reactions catalysed:
ATP + a protein = ADP + a phosphoprotein
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Serine/threonine-protein kinase B-raf Chains: A, B
Molecule details ›
Chains: A, B
Length: 805 amino acids
Theoretical weight: 89.31 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P15056 (Residues: 1-766; Coverage: 100%)
Gene names: BRAF, BRAF1, RAFB1
Sequence domains:
Dual specificity mitogen-activated protein kinase kinase 1 Chain: C
Molecule details ›
Chain: C
Length: 414 amino acids
Theoretical weight: 45.84 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q02750 (Residues: 1-392; Coverage: 100%)
Gene names: MAP2K1, MEK1, PRKMK1
Sequence domains: Protein kinase domain
14_3_3 domain-containing protein Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 247 amino acids
Theoretical weight: 28.11 KDa
Source organism: Spodoptera exigua
UniProt:
  • Canonical: V9P4T4 (Residues: 1-247; Coverage: 100%)
Sequence domains: 14-3-3 protein

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 5.7Å
Relevant EMDB volumes: EMD-20552
Expression system: Spodoptera frugiperda