6pzy

Electron Microscopy
3.17Å resolution

CryoEM derived model of NA-73 Fab in complex with N9 Shanghai2

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Neuraminidase Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 429 amino acids
Theoretical weight: 48.21 KDa
Source organism: Influenza A virus (A/environment/Shanghai/S1439/2013(H7N9))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: S5MF06 (Residues: 37-465; Coverage: 92%)
Gene name: NA
Sequence domains: Neuraminidase
NA-73 fragment antibody heavy chain Chains: C, G, H, I
Molecule details ›
Chains: C, G, H, I
Length: 232 amino acids
Theoretical weight: 24.82 KDa
Source organism: Homo sapiens
Expression system: Cricetinae gen. sp.
NA-73 fragment antibody light chain Chains: D, J, K, L
Molecule details ›
Chains: D, J, K, L
Length: 216 amino acids
Theoretical weight: 22.79 KDa
Source organism: Homo sapiens
Expression system: Cricetinae gen. sp.

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.17Å
Relevant EMDB volumes: EMD-20540
Expression systems:
  • Spodoptera frugiperda
  • Cricetinae gen. sp.