PDBe 6pzk

Electron Microscopy
3.2Å resolution

Cryo-EM Structure of the Respiratory Syncytial Virus Polymerase (L) Protein Bound by the Tetrameric Phosphoprotein (P)

Released:

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
5'-triphospho-mRNA + GDP = diphosphate + guanosine 5'-triphospho-mRNA
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppR-RNA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RNA-directed RNA polymerase L Chain: A
Molecule details ›
Chain: A
Length: 2201 amino acids
Theoretical weight: 254.48 KDa
Source organism: Human respiratory syncytial virus A2
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P28887 (Residues: 1-2165; Coverage: 100%)
Gene name: L
Sequence domains:
Phosphoprotein Chains: B, C, D, E
Molecule details ›
Chains: B, C, D, E
Length: 256 amino acids
Theoretical weight: 29.06 KDa
Source organism: Human respiratory syncytial virus A2
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P03421 (Residues: 1-241; Coverage: 100%)
Gene name: P
Sequence domains: Pneumovirus phosphoprotein

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-20536
Expression system: Spodoptera frugiperda