› 6px4

X-ray diffraction
1.65Å resolution

Crystal structure of the complex between periplasmic domains of antiholin RI and holin T from T4 phage, in H32

Released:
DOI: 10.2210/pdb6px4/pdb
PDB entry 6px4 coloured by chain, front view.
PDB entry 6px4 coloured by chain, side view.
PDB entry 6px4 coloured by chain, top view.
Source organisms:
Primary publication:
The Structural Basis of T4 Phage Lysis Control: DNA as the Signal for Lysis Inhibition.
Krieger IV, Kuznetsov V, Chang JY, Zhang J, Moussa SH, Young RF, Sacchettini JC
J Mol Biol 432 4623-4636 (2020)
PMID: 32562709

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139242 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Antiholin Chains: A, R
Molecule details ›
Chains: A, R
Length: 74 amino acids
Theoretical weight: 8.8 KDa
Source organism: Escherichia phage ECML-134
Expression system: Escherichia coli
UniProt:
  • Canonical: P13304 (Residues: 25-97; Coverage: 100%)
Gene names: 58.6, rI, rIA, tk.-2
Holin Chains: B, T
Molecule details ›
Chains: B, T
Length: 142 amino acids
Theoretical weight: 16.44 KDa
Source organism: Escherichia phage vB_EcoM_NBG2
Expression system: Escherichia coli
UniProt:
  • Canonical: P06808 (Residues: 77-218; Coverage: 65%)
Gene names: rV, t
Sequence domains: Bacteriophage T holin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R32
Unit cell:
a: 116.546Å b: 116.546Å c: 223.384Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.194 0.225
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Towards the Idea of Molecular Brains.
Timsit et al. (2021)
1 more

4 mentions without citation

Implications of AlphaFold2 for crystallographic phasing by molecular replacement.
McCoy et al. (2022)
3 more