› 6pw0

X-ray diffraction
2.5Å resolution

Cytochrome C oxidase delta 6 mutant

Released:
DOI: 10.2210/pdb6pw0/pdb
PDB 6pw0 coloured by chain and viewed from the front.
PDB 6pw0 coloured by chain and viewed from the side.
PDB 6pw0 coloured by chain and viewed from the top.
Source organism: Rhodobacter sphaeroides 2.4.1
Primary publication:
Structural changes at the surface of cytochrome c oxidase alter the proton-pumping stoichiometry.
Berg J, Liu J, Svahn E, Ferguson-Miller S, Brzezinski P
Biochim Biophys Acta Bioenerg 148116 (2019)
PMID: 31733183

Function and Biology Details

Reaction catalysed: 
4 ferrocytochrome c + O(2) + 4 H(+) = 4 ferricytochrome c + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Cytochrome c oxidase subunit 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 560 amino acids
Theoretical weight: 62.53 KDa
Source organism: Rhodobacter sphaeroides 2.4.1
Expression system: Rhodobacter sphaeroides 2.4.1
UniProt:
  • Canonical: Q3J5A7 (Residues: 1-560; Coverage: 99%)
Gene names: RSP_1877, coxI
Sequence domains: Cytochrome C and Quinol oxidase polypeptide I
Cytochrome c oxidase subunit 2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 262 amino acids
Theoretical weight: 29.37 KDa
Source organism: Rhodobacter sphaeroides 2.4.1
Expression system: Rhodobacter sphaeroides 2.4.1
UniProt:
  • Canonical: Q3J5G0 (Residues: 26-281; Coverage: 92%)
Gene names: RSP_1826, coxII
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Ligand GLC 4 x GLC
10 bound ligands:
Ligand OH 2 x OH
Ligand HEA 4 x HEA
Ligand DMU 9 x DMU
Ligand TRD 15 x TRD
Ligand HTH 2 x HTH
Ligand CU 6 x CU
Ligand MG 2 x MG
Ligand CA 2 x CA
Ligand CD 4 x CD
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 124.905Å b: 131.477Å c: 176.601Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.208
Expression system: Rhodobacter sphaeroides 2.4.1

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