› 6puc

X-ray diffraction
1.85Å resolution

Structure of human MAIT A-F7 TCR in complex with human MR1-5-OP-RU

Released:
DOI: 10.2210/pdb6puc/pdb
PDB entry 6puc coloured by chain, front view.
PDB entry 6puc coloured by chain, side view.
PDB entry 6puc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The molecular basis underpinning the potency and specificity of MAIT cell antigens.
Awad W, Ler GJM, Xu W, Keller AN, Mak JYW, Lim XY, Liu L, Eckle SBG, Le Nours J, McCluskey J, Corbett AJ, Fairlie DP, Rossjohn J
Nat Immunol 21 400-411 (2020)
PMID: 32123373

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-231830 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Major histocompatibility complex class I-related gene protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 271 amino acids
Theoretical weight: 31.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q95460 (Residues: 23-292; Coverage: 85%)
Gene name: MR1
Sequence domains:
Beta-2-microglobulin Chains: B, F
Molecule details ›
Chains: B, F
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Human TCR alpha chain Chains: D, G
Molecule details ›
Chains: D, G
Length: 204 amino acids
Theoretical weight: 22.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Human TCR beta chain Chains: E, H
Molecule details ›
Chains: E, H
Length: 246 amino acids
Theoretical weight: 27.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

4 bound ligands:
Ligand Q87 2 x Q87
Ligand GOL 3 x GOL
Ligand CL 2 x CL
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: C2
Unit cell:
a: 217.967Å b: 70.248Å c: 143.634Å
α: 90° β: 104.808° γ: 90°
R-values:
R R work R free
0.16 0.159 0.178
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The Immune Modulating Properties of Mucosal-Associated Invariant T Cells.
Ioannidis et al. (2020)
6 more

6 mentions without citation

Antigen Recognition by MR1-Reactive T Cells; MAIT Cells, Metabolites, and Remaining Mysteries.
Corbett et al. (2020)
5 more