› 6pu6

X-ray diffraction
2.29Å resolution

CobT from Methanocaldococcus jannaschii in complex with Alpha-Ribozole 5'-Phosphate, Nicotinic Acid, and Nicotinic Acid Mononucleotide

Released:
DOI: 10.2210/pdb6pu6/pdb
PDB entry 6pu6 coloured by chain, front view.
PDB entry 6pu6 coloured by chain, side view.
PDB entry 6pu6 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Primary publication:
Structural studies of the phosphoribosyltransferase involved in cobamide biosynthesis in methanogenic archaea and cyanobacteria.
Jeter VL, Schwarzwalder AH, Rayment I, Escalante-Semerena JC
Sci Rep 12 17175 (2022)
PMID: 36229494

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176730 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UPF0284 protein MJ1598 Chains: A, B
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 37.85 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58993 (Residues: 1-350; Coverage: 100%)
Gene name: MJ1598
Sequence domains: Phosphoribosyltransferase

Ligands and Environments

3 bound ligands:
Ligand NCN 1 x NCN
Ligand RBZ 1 x RBZ
Ligand NIO 1 x NIO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 47.58Å b: 139Å c: 54.49Å
α: 90° β: 111.67° γ: 90°
R-values:
R R work R free
0.196 0.193 0.24
Expression system: Escherichia coli

Quick links