› 6psi

Solution NMR

Structural Basis for Client Recognition and Activity of Hsp40 Chaperones

Released:
DOI: 10.2210/pdb6psi/pdb
PDB 6psi coloured by chain and viewed from the front.
PDB 6psi coloured by chain and viewed from the side.
PDB 6psi coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Structural basis for client recognition and activity of Hsp40 chaperones.
Jiang Y, Rossi P, Kalodimos CG
Science 365 1313-1319 (2019)
PMID: 31604242

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chaperone protein DnaJ 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 280 amino acids
Theoretical weight: 31.02 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q56237 (Residues: 1-280; Coverage: 100%)
Gene names: TTHA1489, dnaJ2
Sequence domains:
Alkaline phosphatase Chain: B
Molecule details ›
Chain: B
Length: 471 amino acids
Theoretical weight: 49.49 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00634 (Residues: 1-471; Coverage: 100%)
Gene names: JW0374, b0383, phoA
Sequence domains: Alkaline phosphatase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 22%
Refinement method: molecular dynamics
Chemical shifts: BMR30638  
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Heat Shock Proteins in Benign Prostatic Hyperplasia and Prostate Cancer.
Ratajczak et al. (2022)
10 more