Solution NMR

Structural Basis for Client Recognition and Activity of Hsp40 Chaperones


Function and Biology Details

Reaction catalysed:
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chaperone protein DnaJ 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 280 amino acids
Theoretical weight: 31.02 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q56237 (Residues: 1-280; Coverage: 100%)
Gene names: TTHA1489, dnaJ2
Sequence domains:
Alkaline phosphatase Chain: B
Molecule details ›
Chain: B
Length: 471 amino acids
Theoretical weight: 49.49 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P00634 (Residues: 1-471; Coverage: 100%)
Gene names: JW0374, b0383, phoA
Sequence domains: Alkaline phosphatase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 22%
Refinement method: molecular dynamics
Chemical shifts: BMR30638  
Expression system: Escherichia coli BL21(DE3)