6pqm

Solution NMR

Structural Basis for Client Recognition and Activity of Hsp40 Chaperones

Released:

Function and Biology Details

Reaction catalysed:
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkaline phosphatase; Chaperone protein DnaJ 2 Chain: A
Molecule details ›
Chain: A
Length: 94 amino acids
Theoretical weight: 9.57 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00634 (Residues: 415-430; Coverage: 4%)
  • Canonical: Q56237 (Residues: 153-153, 191-256; Coverage: 24%)
Gene names: JW0374, TTHA1489, b0383, dnaJ2, phoA

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 85%
Refinement method: molecular dynamics
Chemical shifts: BMR30632  
Expression system: Escherichia coli BL21(DE3)