6pq8

X-ray diffraction
2.2Å resolution

Crystal structure of TLA-1 S70G extended spectrum Beta-lactamase in complex with clavulanic acid

Released:

Function and Biology Details

Reaction catalysed:
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 33.34 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X6W1 (Residues: 14-312; Coverage: 99%)
Gene name: tla-1
Sequence domains: Beta-lactamase enzyme family

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P41212
Unit cell:
a: 99.41Å b: 99.41Å c: 99.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.226
Expression system: Escherichia coli BL21(DE3)