› 6pmo

X-ray diffraction
2.66Å resolution

Co-crystal structure of the Geobacillus kaustophilus glyQ T-box riboswitch discriminator domain in complex with tRNA-Gly

Released:
DOI: 10.2210/pdb6pmo/pdb
PDB 6pmo coloured by chain and viewed from the front.
PDB 6pmo coloured by chain and viewed from the side.
PDB 6pmo coloured by chain and viewed from the top.
Source organism: Geobacillus kaustophilus
Primary publication:
Structural basis of amino acid surveillance by higher-order tRNA-mRNA interactions.
Li S, Su Z, Lehmann J, Stamatopoulou V, Giarimoglou N, Henderson FE, Fan L, Pintilie GD, Zhang K, Chen M, Ludtke SJ, Wang YX, Stathopoulos C, Chiu W, Zhang J
Nat. Struct. Mol. Biol. (2019)
PMID: 31740854

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
tRNA-Gly Chain: B
Molecule details ›
Chain: B
Length: 75 nucleotides
Theoretical weight: 24.16 KDa
Source organism: Geobacillus kaustophilus
Expression system: Not provided
T-box riboswitch discriminator Chain: A
Molecule details ›
Chain: A
Length: 66 nucleotides
Theoretical weight: 21.37 KDa
Source organism: Geobacillus kaustophilus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand IR 13 x IR
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 44.851Å b: 139.913Å c: 136.023Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.225 0.273
Expression system: Not provided

Quick links

Citations

8 review citations

Cryo-EM advances in RNA structure determination.
Ma et al. (2022)
7 more