6pih

Electron Microscopy
6.6Å resolution

Hexameric ArnA cryo-EM structure

Released:

Function and Biology Details

Reactions catalysed:
UDP-alpha-D-glucuronate + NAD(+) = UDP-beta-L-threo-pentapyranos-4-ulose + CO(2) + NADH
10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinose = 5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4-formamido-beta-L-arabinose
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bifunctional polymyxin resistance protein ArnA Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 300 amino acids
Theoretical weight: 32.72 KDa
Source organism: Escherichia coli DH5[alpha]
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P77398 (Residues: 1-300; Coverage: 46%)
Gene names: JW2249, arnA, b2255, pmrI, yfbG
Sequence domains:
Bifunctional polymyxin resistance protein ArnA Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 345 amino acids
Theoretical weight: 40.06 KDa
Source organism: Escherichia coli DH5[alpha]
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P77398 (Residues: 317-660; Coverage: 52%)
Gene names: JW2249, arnA, b2255, pmrI, yfbG
Sequence domains: NAD dependent epimerase/dehydratase family

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.6Å
Relevant EMDB volumes: EMD-0456
Expression system: Escherichia coli BL21(DE3)