› 6pdl

X-ray diffraction
2.7Å resolution

Crystal Structure of Hendra Virus Attachment G Glycoprotein in Complex with Receptor Ephrin-B2

Released:
DOI: 10.2210/pdb6pdl/pdb
PDB entry 6pdl coloured by chain, front view.
PDB entry 6pdl coloured by chain, side view.
PDB entry 6pdl coloured by chain, top view.
Source organisms:
Primary publication:
New insights into the Hendra virus attachment and entry process from structures of the virus G glycoprotein and its complex with Ephrin-B2.
Xu K, Chan YP, Rajashankar KR, Khetawat D, Yan L, Kolev MV, Broder CC, Nikolov DB
PLoS One 7 e48742 (2012)
PMID: 23144952

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131624 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (7 distinct):
Glycoprotein G Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 441 amino acids
Theoretical weight: 49.43 KDa
Source organism: Henipavirus hendraense
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: O89343 (Residues: 171-604; Coverage: 72%)
Gene name: G
Sequence domains: Haemagglutinin-neuraminidase
Ephrin-B2 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 141 amino acids
Theoretical weight: 16.1 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P52799 (Residues: 27-167; Coverage: 46%)
Gene names: EFNB2, EPLG5, HTKL, LERK5
Sequence domains: Ephrin

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
1 bound ligand:
Ligand NAG 13 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P1
Unit cell:
a: 75.764Å b: 94.642Å c: 102.522Å
α: 112.86° β: 97.44° γ: 97.56°
R-values:
R R work R free
0.175 0.172 0.227
Expression system: Insect cell expression vector pTIE1

Quick links

Citations

6 review citations

A treatment for and vaccine against the deadly Hendra and Nipah viruses.
Broder et al. (2013)
5 more

4 mentions without citation

Cooperativity mediated by rationally selected combinations of human monoclonal antibodies targeting the henipavirus receptor binding protein.
Doyle et al. (2021)
3 more