6pax

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF THE HUMAN PAX-6 PAIRED DOMAIN-DNA COMPLEX REVEALS A GENERAL MODEL FOR PAX PROTEIN-DNA INTERACTIONS

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Paired box protein Pax-6 Chain: A
Molecule details ›
Chain: A
Length: 133 amino acids
Theoretical weight: 14.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26367 (Residues: 4-136; Coverage: 32%)
Gene names: AN2, PAX6
Sequence domains: 'Paired box' domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
26 NUCLEOTIDE DNA Chain: B
Molecule details ›
Chain: B
Length: 26 nucleotides
Theoretical weight: 8 KDa
Source organism: Homo sapiens
Expression system: Not provided
26 NUCLEOTIDE DNA Chain: C
Molecule details ›
Chain: C
Length: 26 nucleotides
Theoretical weight: 7.98 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 33.84Å b: 61.686Å c: 171.111Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.233 0.256
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided