6pa3

X-ray diffraction
1.65Å resolution

E. coli L-asparaginase II double mutant (T12V,K162T) in complex with L-Asn at pH 7.0

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-asparaginase 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 334 amino acids
Theoretical weight: 35.67 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00805 (Residues: 23-348; Coverage: 100%)
Gene names: JW2924, ansB, b2957
Sequence domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 152.852Å b: 62.99Å c: 141.293Å
α: 90° β: 117.83° γ: 90°
R-values:
R R work R free
0.15 0.149 0.184
Expression system: Escherichia coli BL21(DE3)