› 6p9r

X-ray diffraction
1.75Å resolution

Function and Biology Details

Reaction catalysed: 
A (3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = an [acyl-carrier-protein] + a UDP-3-O-(3-hydroxyacylyl)-N-acetyl-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141513 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 28.95 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A722 (Residues: 1-262; Coverage: 100%)
Gene names: JW0176, b0181, lpxA
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand U20 1 x U20
Ligand PO4 2 x PO4
Ligand DMS 3 x DMS
Ligand O5M 1 x O5M
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P213
Unit cell:
a: 96.556Å b: 96.556Å c: 96.556Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.185
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Structure- and Ligand-Dynamics-Based Design of Novel Antibiotics Targeting Lipid A Enzymes LpxC and LpxH in Gram-Negative Bacteria.
Zhou et al. (2021)
3 more