6p8h

X-ray diffraction
3.19Å resolution

Crystal structure of CDK4 in complex with CyclinD1 and P21

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
G1/S-specific cyclin-D1 Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 28.45 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P24385 (Residues: 19-267; Coverage: 84%)
Gene names: BCL1, CCND1, PRAD1
Sequence domains: Cyclin, N-terminal domain
Cyclin-dependent kinase 4 Chain: B
Molecule details ›
Chain: B
Length: 302 amino acids
Theoretical weight: 33.95 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P11802 (Residues: 2-303; Coverage: 99%)
  • Best match: P11802-2 (Residues: 1-183)
Gene name: CDK4
Sequence domains: Protein kinase domain
Cyclin-dependent kinase inhibitor 1 Chain: C
Molecule details ›
Chain: C
Length: 80 amino acids
Theoretical weight: 9.09 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P38936 (Residues: 9-85; Coverage: 47%)
Gene names: CAP20, CDKN1, CDKN1A, CIP1, MDA6, PIC1, SDI1, WAF1
Sequence domains: Cyclin-dependent kinase inhibitor

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Unit cell:
a: 62.62Å b: 67.98Å c: 185.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.258
Expression system: Spodoptera frugiperda