6p71

X-ray diffraction
2.92Å resolution

X-ray crystal structure of a bacterial reiterative transcription complex of pyrBI promoter

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 35.06 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SHR6 (Residues: 1-315; Coverage: 100%)
Gene names: TTHA1664, rpoA
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1119 amino acids
Theoretical weight: 125.44 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q8RQE9 (Residues: 1-1119; Coverage: 100%)
Gene names: TTHA1813, rpoB
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
Molecule details ›
Chain: D
Length: 1524 amino acids
Theoretical weight: 171 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q8RQE8 (Residues: 1-1524; Coverage: 100%)
Gene names: TTHA1812, rpoC
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: E
Molecule details ›
Chain: E
Length: 99 amino acids
Theoretical weight: 11.53 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q8RQE7 (Residues: 1-99; Coverage: 100%)
Gene names: TTHA1561, rpoZ
Sequence domains: RNA polymerase Rpb6
RNA polymerase sigma factor SigA Chain: F
Molecule details ›
Chain: F
Length: 423 amino acids
Theoretical weight: 48.6 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q72L95 (Residues: 1-423; Coverage: 100%)
Gene names: TT_C0164, rpoD, sigA
Sequence domains:
DNA (5'-D(P*CP*TP*CP*CP*CP*GP*GP*CP*AP*AP*AP*TP*TP*GP*T)-3') Chain: G
Molecule details ›
Chain: G
Length: 21 nucleotides
Theoretical weight: 6.36 KDa
DNA (5'-D(*TP*AP*TP*AP*AP*TP*CP*GP*AP*GP*CP*CP*GP*GP*GP*A)-3') Chain: H
Molecule details ›
Chain: H
Length: 27 nucleotides
Theoretical weight: 8.36 KDa
RNA (5'-R(P*AP*AP*UP*U)-3') Chain: I
Molecule details ›
Chain: I
Length: 4 nucleotides
Theoretical weight: 1.23 KDa

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: C2
Unit cell:
a: 187.506Å b: 100.662Å c: 296.304Å
α: 90° β: 98.32° γ: 90°
R-values:
R R work R free
0.212 0.211 0.248