› 6p1a

X-ray diffraction
2.84Å resolution

Transcription antitermination factor Q21 in complex with Q21-binding-element DNA

Released:
DOI: 10.2210/pdb6p1a/pdb
PDB 6p1a coloured by chain and viewed from the front.
PDB 6p1a coloured by chain and viewed from the side.
PDB 6p1a coloured by chain and viewed from the top.
Source organism: Phage 21
Primary publication:
Structural basis of Q-dependent antitermination.
Yin Z, Kaelber JT, Ebright RH
Proc. Natl. Acad. Sci. U.S.A. (2019)
PMID: 31455742

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero trimer
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Q protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 162 amino acids
Theoretical weight: 18.67 KDa
Source organism: Phage 21
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9XJQ6 (Residues: 2-23, 24-161; Coverage: 99%)
Gene name: Q
Sequence domains: Phage antitermination protein Q
DNA (5'-D(P*CP*TP*CP*AP*TP*TP*GP*AP*GP*CP*AP*AP*AP*TP*GP*AP*GP*CP*AP*AP*G)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 21 nucleotides
Theoretical weight: 6.48 KDa
DNA (5'-D(*CP*TP*TP*GP*CP*TP*CP*AP*TP*TP*TP*GP*CP*TP*CP*AP*AP*TP*GP*AP*G)-3') Chains: E, G
Molecule details ›
Chains: E, G
Length: 21 nucleotides
Theoretical weight: 6.4 KDa

Ligands and Environments

2 bound ligands:
Ligand EDO 3 x EDO
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P21212
Unit cell:
a: 69.573Å b: 263.224Å c: 56.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.231 0.275
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Coupled Transcription-Translation in Prokaryotes: An Old Couple With New Surprises.
Irastortza-Olaziregi et al. (2020)
2 other articles

1 mention without citation

Structural basis of Q-dependent antitermination.
Yin et al. (2019)