› 6oyu

X-ray diffraction
2.95Å resolution

Structure of an ancestral-reconstructed cytochrome P450 1B1 with alpha-naphthoflavone

Released:
DOI: 10.2210/pdb6oyu/pdb
PDB entry 6oyu coloured by chain, front view.
PDB entry 6oyu coloured by chain, side view.
PDB entry 6oyu coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Structure of an ancestral mammalian family 1B1 cytochrome P450 with increased thermostability.
Bart AG, Harris KL, Gillam EMJ, Scott EE
J Biol Chem 295 5640-5653 (2020)
PMID: 32156703

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167224 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 1B1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 493 amino acids
Theoretical weight: 55.35 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand BHF 8 x BHF
Ligand HEM 2 x HEM
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: I4
Unit cell:
a: 164.641Å b: 164.641Å c: 96.301Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.199 0.258
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Engineering functional thermostable proteins using ancestral sequence reconstruction.
Thomson et al. (2022)
1 more

2 mentions without citation

Structure of an ancestral mammalian family 1B1 cytochrome P450 with increased thermostability.
Bart et al. (2020)
1 more