› 6otc

X-ray diffraction
1.7Å resolution

Synthetic Fab bound to Marburg virus VP35 interferon inhibitory domain

Released:
DOI: 10.2210/pdb6otc/pdb
PDB entry 6otc coloured by chain, front view.
PDB entry 6otc coloured by chain, side view.
PDB entry 6otc coloured by chain, top view.
Source organisms:
Primary publication:
Inhibition of Marburg Virus RNA Synthesis by a Synthetic Anti-VP35 Antibody.
Amatya P, Wagner N, Chen G, Luthra P, Shi L, Borek D, Pavlenco A, Rohrs H, Basler CF, Sidhu SS, Gross ML, Leung DW
ACS Infect Dis 5 1385-1396 (2019)
PMID: 31120240

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232126 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Polymerase cofactor VP35 Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.13 KDa
Source organism: Lake Victoria marburgvirus - Popp
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03039 (Residues: 204-329; Coverage: 38%)
Gene name: VP35
Sequence domains: Filoviridae VP35
sFab H3 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 240 amino acids
Theoretical weight: 25.83 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
sFab H3 light chain Chain: L
Molecule details ›
Chain: L
Length: 232 amino acids
Theoretical weight: 25.27 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 57.827Å b: 97.422Å c: 110.325Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.233
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Developing Recombinant Antibodies by Phage Display Against Infectious Diseases and Toxins for Diagnostics and Therapy.
Roth et al. (2021)
3 more

2 mentions without citation

Inhibition of Marburg Virus RNA Synthesis by a Synthetic Anti-VP35 Antibody.
Amatya et al. (2019)
1 more