6oq6

X-ray diffraction
2.97Å resolution

Structure of the pore forming fragment of Clostridium difficile toxin B in complex with VHH 5D

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Toxin B Chain: A
Molecule details ›
Chain: A
Length: 363 amino acids
Theoretical weight: 40.54 KDa
Source organism: Clostridioides difficile
Expression system: Escherichia coli
UniProt:
  • Canonical: P18177 (Residues: 1071-1432; Coverage: 15%)
Gene names: tcdB, toxB
5D Chain: D
Molecule details ›
Chain: D
Length: 153 amino acids
Theoretical weight: 16.86 KDa
Source organism: Camelidae
Expression system: Escherichia coli

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P6122
Unit cell:
a: 169.82Å b: 169.82Å c: 79.12Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.26 0.259 0.274
Expression system: Escherichia coli