6omf

Electron Microscopy
3.26Å resolution

CryoEM structure of SigmaS-transcription initiation complex with activator Crl

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (8 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 239 amino acids
Theoretical weight: 26.46 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Z4 (Residues: 1-234; Coverage: 71%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
Molecule details ›
Chain: D
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8T7 (Residues: 1-1407; Coverage: 100%)
Gene names: JW3951, b3988, rpoC, tabB
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: E
Molecule details ›
Chain: E
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A800 (Residues: 1-91; Coverage: 100%)
Gene names: JW3624, b3649, rpoZ
Sequence domains: RNA polymerase Rpb6
RNA polymerase sigma factor RpoS Chain: F
Molecule details ›
Chain: F
Length: 331 amino acids
Theoretical weight: 38.07 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: S5ZIY8 (Residues: 1-330; Coverage: 100%)
Gene names: A3104_08620, A3S30_20665, A3T81_15930, A3U32_19410, A3V03_13255, A3V89_13725, A3W57_16010, A3W75_13615, A3X15_14490, A3X55_09355, A3Y76_11425, A4N07_06640, A4O05_06635, A4O41_11345, A4R48_05500, A6D61_06590, AAA76_13245, AAB27_19985, AAB79_09970, AAC35_15970, ADQ28_09325, AF497_15435, AGM99_07600, AHN93_09105, AKH62_10615, AL144_10200, AL168_09730, AL184_11045, AQ530_14025, AU613_08580, AVA38_07700, AVC05_02015, AVL16_05870, AWT30_06860, AXX99_12025, B1265_07360, B1398_07445, B1642_13675, B1P38_03420, B2E31_15455, B4V59_13555, B4W90_04345, B6362_07540, B8Y16_10650, B8Z46_07780, B9C90_09735, B9C96_07565, B9M14_11930, B9O84_04720, BBQ66_03515, BIC00_01870, BIC13_10380, BK110_07550, BKM50_12450, BMS46_04395, BMU56_05345, BZ203_09310, BZZ88_18535, C5W43_12340, CA117_08505, CB102_08280, CB119_04025, CB198_02275, CB380_14595, CB535_13735, CB570_08670, CB646_10800, CBM67_16610, CBM76_15005, CBZ90_11580, CC339_06135, CC403_06375, CC453_06360, CC652_14770, CC971_01640, CCP17_12515, CDZ72_04005, CE70_12025, CED07_07500, CEQ70_02030, CFF58_10915, CFF59_06065, CHN22_06260, CIX60_02050, CPS79_11280, CQO33_16185, CSG22_12115, CVR97_12745, D4361_10085, D4387_15235, D4422_13060, D5823_15740, D5N86_09065, D5N95_10325, D5O82_16535, D5P17_16625, D5X47_15280, D5Y28_18615, D6422_15720, D6J79_17000, D8S24_11245, DLB93_11520, DLR28_17070, DMI89_17455, DMO92_02765, DN165_11150, DNB97_00870, DNM27_05685, DNZ37_15470, DO533_06145, DP680_15215, DPB42_03875, DPD91_09315, DPF41_13125, DPF68_13905, DPS76_00535, DQD22_04655, DQR44_14110, DRM14_13690, DRR75_02465, DRT38_02000, DRT61_15450, DRV05_05665, DSF94_01780, DSG41_06775, DTF68_01780, DU071_02715, DU223_03870, DU657_08120, DU879_12870, DUV75_06795, DWU22_21745, DY580_03955, DYM27_15210, E0935_16900, E1A11_14085, E6W45_11385, EBD14_10870, EBK21_12590, EC404_10280, EEQ30_03285, EER35_13550, EHB09_17780, EL822_10640, ELS01_13825, EPB30_04780, EQG93_17640, EVY71_02365, EW905_11540, F0D96_04765, F2P00_05860, F9G02_11840, F9O44_07490, FE758_05875, FEM52_10740, FGZ46_12960, FJM64_15970, FQC24_10885, FQX78_01010, G0038_02390, G0040_20470, G0042_02270, G0045_07430, G0047_15345, G0048_07425, G0051_15765, G0052_07060, G0059_10230, G0061_06585, G0062_07115, G0063_17735, G0067_07610, G0069_07185, G0070_05450, G0071_14320, G0072_08435, G0074_06535, G0076_02700, G0077_07355, G0080_07185, G0084_14375, G0086_07425, G0087_07425, G0088_06535, G0089_12535, G0090_12220, G0094_10135, G0100_07425, G0101_06035, G0102_06310, G0111_05690, G0113_02470, G0117_07500, G0123_07425, G0124_07175, G0148_12400, G0157_06840, G0170_11445, G0A28_10920, G0A32_10920, G0A39_10690, G0A43_11170, G0A44_12605, G0A50_10385, G0A51_14490, G0A52_10960, G0A53_10810, G0A56_15975, G0A58_12790, G0A60_07615, G0A61_13100, G0A63_08705, G0A66_11200, G0A67_11450, G0A68_08910, G0A70_13390, G0A73_12605, G0A76_11015, G0A79_16260, G0A92_17240, G0A97_13500, G0A99_08440, G0B05_05460, G0B08_15375, G0B12_10160, G0B28_19325, G0B96_08670, G0C04_05710, G0C34_15175, G0E15_10065, G0E20_07335, G0G84_03800, G0J24_12125, G0J26_16050, G0J27_01175, G0J28_13720, G0J31_14600, G0J33_15430, G0J34_08095, G0J36_11130, G0J37_13285, G0J40_11130, G0J43_10610, G0J44_04740, G0J45_11085, G0J46_11730, G0J47_10945, G0J49_11750, G0J50_16155, G0J51_14590, G0J53_14080, G0J55_12190, G0J58_12925, G0J59_09965, G0J62_11350, G0J65_01725, G0J66_13875, G0J67_14700, G0J69_10525, G0J71_12725, G0J73_05770, G0J76_10950, G0J79_05245, G0J81_24805, G0J82_13650, G0J85_14455, G0J89_06550, G0J92_15030, G0J94_01720, G0J96_01720, G0J97_09105, G0K00_08795, G0K02_04570, G0K03_08885, G0K04_01930, G0K05_04455, G0K07_17960, G0K10_02515, G0K13_15275, G0K15_14145, G0K16_01725, G0K18_13960, G0K19_10645, G0K20_16855, G0K23_11440, G0K25_16500, G0K26_06945, G0K30_01925, G0K31_12070, G0K32_01750, G0K33_16480, G0K37_14140, G0K38_04650, G0K39_02675, G0K41_16150, G0K42_01725, G0K44_07315, G0K46_13355, G0K47_11845, G0K48_10195, G0K49_03575, G0K52_01720, G0K53_02265, G0K56_02265, G0K58_04300, G0K59_06980, G0K61_01720, G0K65_15650, G0K68_09885, G0K70_12845, G0K72_14835, G0K74_06495, G0K75_05295, G0K78_15240, G0K80_13760, G0K83_01725, G0K84_04875, G0K85_12750, G0K89_003540, G0K90_003456, G0K94_003812, G0K95_003938, G0L00_001918, G0L02_003915, G0L03_00730, G0L06_20805, G0L07_23470, G0L10_20885, G0L14_05325, G0L15_17590, G0L18_20100, G0L19_20895, G0L20_07305, G0L24_10430, G0L25_10100, G0L31_13815, G0L32_05230, G0L35_19820, G0L36_07995, G0L37_08815, G0L38_09745, G0L40_04770, G0L42_01760, G0L51_09080, G0L55_09065, G0L59_10150, G0L62_10970, G0L65_08420, G0L67_07590, G0L70_10135, G0L73_15520, G0L76_10970, G0L77_12735, G0L78_08060, G0L79_10440, G0L83_03260, G0L85_06535, G0L86_002027, G0L88_08790, G0L89_12485, G0L91_09385, G0L93_07225, G0L96_12105, G0L98_11925, G0M00_09670, G0M02_10755, G0M05_05885, G0M06_001964, G0M10_02875, G0M13_001956, G0M14_08115, G0M18_000118, G0M22_001806, G0M25_001631, G0M26_10940, G0M27_10370, G0M28_02930, G0M29_002001, G0M30_11425, G0M33_06920, G0M35_15425, G0M36_10700, G0M38_11125, G0M39_06315, G0M40_10210, G0M41_06490, G0M45_06160, G0M46_001484, G0M48_001270, G0M51_11650, G0M53_07300, G0M55_09475, G0M56_10370, G0M58_11180, G0M63_09320, G0M65_09685, G0M67_10580, G0N45_10825, G0N48_10365, G0N51_04640, G0N53_06980, G0N55_05995, G0N57_02250, G0N58_13840, G0N59_07590, G0N60_08335, G0N62_10770, G0N64_05790, G0N65_11665, G0N66_08320, G0N67_04245, G0N71_09160, G0N75_07600, G0N78_02190, G0N82_02385, G0N84_13760, G0N85_06100, G0N86_08235, G0N88_16335, G0N89_07765, G0N90_06135, G0N92_06655, G0N94_11705, G0N95_08495, G0N98_17285, G0N99_15840, G0O03_04120, G0O10_13870, G0O14_07960, G0O15_02185, G0O18_06420, G0O19_07435, G0O20_14820, G0O22_04380, G0O25_11475, G0O27_05995, G0O31_06540, G0O32_02755, G0O36_05750, G0O37_01910, G0O39_07565, G0O40_05860, G0O41_06410, G0O42_13355, G0O43_07190, G0O44_07500, G0O47_14305, G0O52_06605, G0O55_05710, G0O57_11965, G0O58_06645, G0O59_12520, G0O60_05995, G0O63_12780, G0O66_02190, G0O68_13710, G0O70_07185, G0O71_07190, G0O74_02110, G0O75_14415, G0O77_07110, G0O78_06545, G0O80_05995, G0O81_12135, G0O82_08640, G0O84_07185, G0O85_10565, G0O86_02185, G0O87_17140, G0O88_09405, G0O89_08015, G0O92_07260, G0O93_10315, G0O94_02190, G0O97_04960, G0O99_07370, G0P00_15860, G0P01_04365, G0P02_04990, G0P05_02185, G0P06_06715, G0P08_04995, G0P12_14165, G0P13_13310, G0P17_04555, G0P18_02390, G0P19_04070, G0P24_18725, G0P26_07190, G0P28_07260, G0P30_06920, G0P31_07185, G0P36_07750, G0P37_20740, G0P41_05830, G0P44_07730, G0P45_11935, G0P48_16785, G0P49_15490, G0P52_07320, G0P53_20285, G0P56_06540, G0P57_07190, G0P58_12480, G0P63_20700, G0P65_07945, G0P67_02185, G0P68_01635, G0P69_19535, G0P73_20740, G0P75_07665, G0P76_10140, G1N61_13890, G1N64_07295, G1N66_07285, G1N68_07290, G1N71_07305, G1N72_07290, G1N86_07295, G1N87_07310, G1N91_08015, G1O00_06405, G1O02_07300, G1O04_07300, G1O05_07285, G1O08_07280, G1O10_06595, G1O12_07305, G1O16_07300, G1O17_07370, G1O18_07300, G1O20_07295, G1O23_07300, G1O25_07285, G1O26_07295, G1O27_07365, G1O28_07370, G1O29_07360, G1O32_07285, G1O34_07365, G1O38_07705, G1O40_07305, G1O43_07290, G1O46_04460, G1O48_07380, G1O49_07380, G1O51_07295, G1O53_07360, G1O62_07300, G1O63_07285, G1O65_07380, G1O67_05630, G1O68_07295, G1O69_07295, G1O71_05630, G1O72_14165, G1O76_07380, G1O77_07295, G1O80_07375, G1O81_07290, G1O83_07365, G1O84_07375, G1O87_07380, G1O88_07370, G1O89_07305, G1O90_07375, G1O93_07370, G1O94_07365, G1O96_07370, G1P02_07365, G1P03_13810, G1P06_07355, G1P09_05095, G1P10_07305, G1P12_07370, G1P14_11585, G1P15_04450, G1P17_07380, G1P19_07370, G1P23_07375, G1P24_07290, G1P25_14165, G1P26_07290, G1P29_13795, G1P31_07360, G1P35_07295, G1P36_07290, G1P37_13740, G1P40_04535, G1P44_07295, G1P45_07375, G1P47_07365, G1P48_07375, G1P51_07375, G1P52_13740, G1P53_13740, G1P54_07375, G1P55_07375, G1P56_07360, G1P57_13900, G1P58_07365, G1P59_07380, G1P61_04715, G1P64_07380, G1P67_07370, G1P69_07285, G1P72_05630, G1P75_07305, G1P76_09110, G1P78_08395, G1P83_12760, G1P84_07300, G1P87_07370, G1P90_07125, G1P91_07365, G1Q03_07290, G1Q08_06465, G1Q67_07375, G1Q78_13025, G1Q81_13650, G1Q83_07375, G1Q84_07030, G1Q85_12790, G1Q86_07290, G1Q88_04460, G1Q90_07360, G1Q91_07300, G1Q93_10585, G1Q96_07265, G1Q98_07300, G1Q99_10345, G1R01_07290, G1R02_06400, G1R03_07370, G1R04_07310, G1R08_09770, G1R13_06855, G1R15_07820, G1R20_05250, G1R21_07060, G1R22_06475, G1R23_10990, G1R27_07270, G1R28_07380, G1R29_07380, G1R30_07375, G1R31_07575, G1R36_07380, G1R38_07290, G1R40_06470, G1R42_04470, G1R44_07370, G1R45_05540, G1R47_06905, G1R48_10375, G1R51_07280, G1R52_16680, G1R53_07365, G1R63_07375, G1R69_12275, G1R87_06905, G1R93_07285, G1S02_07375, G2203_14350, G2212_08385, G2218_02585, G2221_10845, G2279_01985, G2290_02270, G2793_09525, G2918_07675, G2951_04820, G3221_001909, G3230_001124, G3231_001037, G3247_003995, G3248_000593, G3254_001970, G3263_000438, G3270_001784, G3275_002247, G3312_002375, G3336_000130, G3357_002403, G3369_003967, G3433_002268, G3460_001588, G3464_003071, G3593_001476, G3A35_14500, G3V06_003005, G3V14_001355, G3V17_002309, G3V21_003243, G3V56_002045, G3V57_001541, G3X03_002846, G4189_002294, G4190_002792, G4192_002937, G4198_003142, G4201_000201, G4202_001813, G4A01_000450, G4A73_001005, G4A83_000381, G4A85_002784, G4A87_002262, G4B68_002795, G4B72_001904, G4B74_000836, G4C74_002336, G4D32_001467, G4D46_002810, G4F88_19165, G4F89_04885, G4F91_04645, G4F92_19490, G4G47_001736, G4G62_000606, G4G67_003789, G4G68_001768, G4G75_003029, G4G76_001061, G4G79_001120, G4G97_000694, G4H00_001211, G4H04_000420, G4H07_001776, G4H18_002412, G4H21_002924, G4H24_002412, G4H63_001554, G4I66_001558, G4J07_001521, G4J08_003869, G4J11_001667, G4J12_002489, G4J18_001174, G4J20_000326, G4J37_001589, G4J39_001225, G4J41_000837, G4J45_001003, G4J90_002264, G4K02_001758, G4K03_002291, G4O54_001104, G4O56_002442, G4O59_003067, G4O60_003310, G4O67_001719, G4O69_002902, G4P29_003429, G4P83_000866, G4P85_001566, G4P89_001341, G4P91_000749, G4P93_001633, G4Q12_001680, G4Q28_002064, G4Q31_002064, G4Q50_002681, G4Q52_002706, G4Q59_001503, G4Q60_002091, G4Q63_000376, G4Q67_000854, G4Q94_000769, G4R01_001496, G4R02_001792, G4R15_001519, G4R16_001803, G4W68_001753, G4W73_001009, G4W86_002120, G4W87_002353, G4W88_002257, G4W91_002461, G4Y10_002177, G9269_001699, G9302_001211, G9304_002926, G9305_000901, G9309_001434, G9313_002697, G9314_000578, G9367_002623, G9381_001480, G9C24_003583, G9C41_002901, G9C46_003697, G9C47_002691, G9C49_003014, G9C57_002513, G9C64_000427, G9G03_001997, G9G04_001560, G9G34_002757, G9G36_002841, G9G45_003099, G9G50_003760, G9G62_000397, G9W19_003090, G9W28_000377, G9W45_003665, G9W52_001899, G9W63_002870, G9W65_002071, G9W79_001717, G9W95_002072, G9W96_002141, G9X40_001745, GB021_05920, GB040_10345, GB055_12690, GB076_17380, GB114_14980, GB120_16390, GB122_12045, GB131_10380, GB139_13075, GB171_11570, GB209_08865, GB224_09950, GB238_09825, GB280_13160, GB321_12955, GB331_09480, GB339_05365, GB342_11310, GB368_05545, GB372_17605, GB416_08010, GB452_14375, GB459_02185, GB466_02185, GB505_13740, GB510_13515, GB551_08075, GB567_09650, GB645_08775, GBS44_07745, GBS58_02385, GBV53_05110, GBV54_09570, GBV60_06225, GBW03_05595, GBW44_06720, GBW52_11520, GBW76_07885, GBX12_13300, GBX20_07640, GBX46_06085, GBX55_04505, GBX64_11490, GBY13_01905, GBY23_09320, GBZ51_00845, GBZ55_08205, GCZ80_07070, GEZ01_15585, GJE27_08685, GNA59_003196, GNA97_000439, GNB86_002427, GNB92_002779, GNC11_002816, GNC75_002352, GNC95_002220, GND07_002347, GT380_04050, GTH60_13430, GTH63_06690, GTH66_10505, GTH67_04650, GTH68_02390, GTH70_09205, GTH72_10385, GTH73_13510, GTH75_14315, GTH77_14230, GTH78_04995, GTH79_10085, GTH81_18300, GTH85_07120, GTH87_05995, GTH89_02275, GTH90_05600, GTH91_04845, GTH93_07185, GTH94_14600, GTH99_02270, GXC56_07485, GXG40_07485, GYI58_11615, GYI62_001493, GYI77_13010, GYJ04_04930, GYJ24_03330, GYJ27_05780, GYJ28_000413, GYJ30_07120, GYJ32_07350, GYJ53_07370, GYJ59_07785, GYJ60_07360, KP44_07735, NG06_09715, R035_04365, STMLT2P22_CBEKMEGD_03699, Z700_09755, ZV33_12595, ZX03_03925, ZY40_20480, rpoS
Sequence domains:
Sigma factor-binding protein Crl Chain: J
Molecule details ›
Chain: J
Length: 136 amino acids
Theoretical weight: 16.11 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7CR52 (Residues: 1-133; Coverage: 100%)
Gene names: STM0319, crl
Sequence domains: Sigma factor-binding transcriptional regulator Crl
Template DNA strand Chain: T
Molecule details ›
Chain: T
Length: 66 nucleotides
Theoretical weight: 20.33 KDa
Non-template DNA strand Chain: N
Molecule details ›
Chain: N
Length: 66 nucleotides
Theoretical weight: 20.36 KDa

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.26Å
Relevant EMDB volumes: EMD-20090
Expression system: Escherichia coli