6oij

Electron Microscopy
3.3Å resolution

Muscarinic acetylcholine receptor 1-G11 protein complex

Released:
Source organisms:
Related structures: EMD-20078

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein subunit alpha-11 Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 41.27 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A3B3ITX3 (Residues: 2-30; Coverage: 48%)
  • Canonical: P29992 (Residues: 7-7, 37-359; Coverage: 90%)
Gene names: GA11, GNA11, GNAI1
Muscarinic acetylcholine receptor M1 Chain: R
Molecule details ›
Chain: R
Length: 363 amino acids
Theoretical weight: 41.24 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P11229 (Residues: 2-460; Coverage: 75%)
Gene name: CHRM1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Antibody fragment Chain: H
Molecule details ›
Chain: H
Length: 256 amino acids
Theoretical weight: 27.34 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
Structure domains: Immunoglobulins
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Structure domains: Transducin (heterotrimeric G protein), gamma chain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-20078
Expression systems:
  • Trichoplusia ni
  • Spodoptera frugiperda