› 6oh3

X-ray diffraction
2.75Å resolution

X-ray crystal structure of the mouse CMP-sialic acid transporter in complex with CMP-sialic acid, by lipidic cubic phase

Released:
DOI: 10.2210/pdb6oh3/pdb
PDB entry 6oh3 coloured by chain, front view.
PDB entry 6oh3 coloured by chain, side view.
PDB entry 6oh3 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for mammalian nucleotide sugar transport.
Ahuja S, Whorton MR
Elife 8 (2019)
PMID: 30985278

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178885 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CMP-sialic acid transporter Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 35.85 KDa
Source organism: Mus musculus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q61420 (Residues: 1-322; Coverage: 96%)
Gene name: Slc35a1
Sequence domains: Nucleotide-sugar transporter

Ligands and Environments

1 bound ligand:
Ligand NCC 1 x NCC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 140.096Å b: 50.123Å c: 50.419Å
α: 90° β: 109.25° γ: 90°
R-values:
R R work R free
0.257 0.256 0.279
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

Polysialic Acid in the Immune System.
Villanueva-Cabello et al. (2021)
1 more

3 mentions without citation

Expanding the repertoire of methanocarba nucleosides from purinergic signaling to diverse targets.
Jacobson et al. (2021)
2 more