› 6ogx

X-ray diffraction
2.77Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211244 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Fab 1 Heavy Chain Chain: C
Molecule details ›
Chain: C
Length: 225 amino acids
Theoretical weight: 24.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Fab1 Light Chain Chain: D
Molecule details ›
Chain: D
Length: 214 amino acids
Theoretical weight: 23.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Tumor necrosis factor receptor superfamily member 4 Chain: G
Molecule details ›
Chain: G
Length: 163 amino acids
Theoretical weight: 17.7 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P43489 (Residues: 29-170; Coverage: 57%)
Gene names: TNFRSF4, TXGP1L
Sequence domains: TNFR/NGFR cysteine-rich region
Fab2 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 222 amino acids
Theoretical weight: 23.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Fab2 light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: I222
Unit cell:
a: 109.765Å b: 125.249Å c: 197.488Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.254
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

Quick links

Citations

8 review citations

Avidity in antibody effector functions and biotherapeutic drug design.
Oostindie et al. (2022)
7 more

1 mention without citation

Tetravalent biepitopic targeting enables intrinsic antibody agonism of tumor necrosis factor receptor superfamily members.
Yang et al. (2019)