6oe7

X-ray diffraction
2.2Å resolution

Crystal structure of HMCES cross-linked to DNA abasic site

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Abasic site processing protein HMCES Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96FZ2 (Residues: 2-270; Coverage: 76%)
Gene names: C3orf37, DC12, HMCES, SRAPD1
Structure domains: SOS response associated peptidase-like
DNA (5'-D(*GP*TP*CP*TP*GP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 6 nucleotides
Theoretical weight: 1.84 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*CP*AP*GP*AP*CP*GP*TP*(DRZ)P*GP*TP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.55 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Unit cell:
a: 55.509Å b: 51.534Å c: 149.72Å
α: 90° β: 92.72° γ: 90°
R-values:
R R work R free
0.195 0.194 0.216
Expression systems:
  • Escherichia coli
  • Not provided