6ocw

X-ray diffraction
2.6Å resolution

Crystal Structure of Mycobacterium tuberculosis Proteasome in Complex with Phenylimidazole-based Inhibitor A85

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proteasome subunit alpha Chains: A, B, C, D, E, F, G, O, P, Q, R, S, T, U
Molecule details ›
Chains: A, B, C, D, E, F, G, O, P, Q, R, S, T, U
Length: 240 amino acids
Theoretical weight: 25.97 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHU1 (Residues: 10-248; Coverage: 96%)
Gene names: Rv2109c, prcA
Sequence domains: Proteasome subunit
Proteasome subunit beta Chains: H, I, J, K, L, M, N, V, W, X, Y, Z, a, b
Molecule details ›
Chains: H, I, J, K, L, M, N, V, W, X, Y, Z, a, b
Length: 234 amino acids
Theoretical weight: 24.45 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHT9 (Residues: 58-291; Coverage: 80%)
Gene names: Rv2110c, prcB

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 120.183Å b: 198.647Å c: 166.355Å
α: 90° β: 103.58° γ: 90°
R-values:
R R work R free
0.188 0.186 0.241
Expression system: Escherichia coli