6oa8

X-ray diffraction
1.37Å resolution

Superfolder Green Fluorescent Protein with 4-cyano-L-phenylalanine at the chromophore (position 66)

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.91 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli str. K-12 substr. DH10B
UniProt:
  • Canonical: P42212 (Residues: 3-238; Coverage: 98%)
Gene name: GFP
Sequence domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P4122
Unit cell:
a: 55.266Å b: 55.266Å c: 166.483Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.188
Expression system: Escherichia coli str. K-12 substr. DH10B