› 6oa8

X-ray diffraction
1.37Å resolution

Superfolder Green Fluorescent Protein with 4-cyano-L-phenylalanine at the chromophore (position 66)

Released:
DOI: 10.2210/pdb6oa8/pdb
PDB 6oa8 coloured by chain and viewed from the front.
PDB 6oa8 coloured by chain and viewed from the side.
PDB 6oa8 coloured by chain and viewed from the top.
Source organism: Aequorea victoria
Primary publication:
Structural and spectrophotometric investigation of two unnatural amino-acid altered chromophores in the superfolder green fluorescent protein
Olenginski GM, Piacentini J, Harris DR, Runko N, Papoutsis BM, Alter JR, Hess KR, Brewer SH, Phillips-Piro CM
Acta Crystallogr. D Biol. Crystallogr. (2021)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.91 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli str. K-12 substr. DH10B
UniProt:
  • Canonical: P42212 (Residues: 3-238; Coverage: 98%)
Gene name: GFP
Sequence domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
Ligand EDO 3 x EDO
Ligand SO4 8 x SO4
Ligand NA 4 x NA
1 modified residue:
Ligand M3V 1 x M3V

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P4122
Unit cell:
a: 55.266Å b: 55.266Å c: 166.483Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.188
Expression system: Escherichia coli str. K-12 substr. DH10B

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