X-ray diffraction
2Å resolution

Nitrogenase MoFeP mutant S188A from Azotobacter vinelandii in the dithionite reduced state after redox cycling


Function and Biology Details

Reaction catalysed:
8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nitrogenase molybdenum-iron protein alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 492 amino acids
Theoretical weight: 55.36 KDa
Source organism: Azotobacter vinelandii
  • Canonical: P07328 (Residues: 1-492; Coverage: 100%)
Gene name: nifD
Sequence domains: Nitrogenase component 1 type Oxidoreductase
Nitrogenase molybdenum-iron protein beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 523 amino acids
Theoretical weight: 59.52 KDa
Source organism: Azotobacter vinelandii
  • Canonical: P07329 (Residues: 1-523; Coverage: 100%)
Gene name: nifK
Sequence domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 77.437Å b: 130.413Å c: 107.751Å
α: 90° β: 109.09° γ: 90°
R R work R free
0.19 0.186 0.229