› 6o6e

X-ray diffraction
2.14Å resolution

Crystal structure of PltF trapped with PltL using a proline adenosine vinylsulfonamide inhibitor

Released:
DOI: 10.2210/pdb6o6e/pdb
PDB entry 6o6e coloured by chain, front view.
PDB entry 6o6e coloured by chain, side view.
PDB entry 6o6e coloured by chain, top view.
Source organism: Pseudomonas protegens Pf-5
Primary publication:
Dynamic visualization of type II peptidyl carrier protein recognition in pyoluteorin biosynthesis.
Corpuz JC, Podust LM, Davis TD, Jaremko MJ, Burkart MD
RSC Chem Biol 1 8-12 (2020)
PMID: 33305272

Function and Biology Details

Reaction catalysed: 
(1a) ATP + L-proline = diphosphate + (L-prolyl)adenylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175617 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
L-proline--[L-prolyl-carrier protein] ligase Chain: B
Molecule details ›
Chain: B
Length: 513 amino acids
Theoretical weight: 56.4 KDa
Source organism: Pseudomonas protegens Pf-5
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q4KCY5 (Residues: 1-498; Coverage: 100%)
Gene names: PFL_2792, pltF
Sequence domains:
Carrier domain-containing protein Chain: E
Molecule details ›
Chain: E
Length: 96 amino acids
Theoretical weight: 11.14 KDa
Source organism: Pseudomonas protegens Pf-5
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q4KCZ1 (Residues: 1-86; Coverage: 98%)
Gene names: PFL_2786, pltL
Sequence domains: Phosphopantetheine attachment site

Ligands and Environments

4 bound ligands:
Ligand FMT 13 x FMT
Ligand TRS 1 x TRS
Ligand MLA 2 x MLA
Ligand I5M 1 x I5M
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P3221
Unit cell:
a: 170.55Å b: 170.55Å c: 64.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.214 0.26
Expression system: Escherichia coli BL21

Quick links

Citations

6 citation in other articles

FRET Monitoring of a Nonribosomal Peptide Synthetase Elongation Module Reveals Carrier Protein Shuttling between Catalytic Domains.
Rüschenbaum et al. (2022)
5 more

2 mentions without citation

Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains.
Corpuz et al. (2022)
1 more