6o1o

Electron Microscopy
3.8Å resolution

Cryo-EM structure of the T. thermophilus Csm complex bound to target ssRNA

Released:
Source organism: Thermus thermophilus
Related structures: EMD-0454

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (6 distinct):
Csm1 Chain: A
Molecule details ›
Chain: A
Length: 716 amino acids
Theoretical weight: 60.95 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
Csm4 Chains: B, G, H
Molecule details ›
Chains: B, G, H
Length: 279 amino acids
Theoretical weight: 23.76 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
Csm3 Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 218 amino acids
Theoretical weight: 18.57 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
Csm2 Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 118 amino acids
Theoretical weight: 10.06 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
RNA (36-MER) Chain: M
Molecule details ›
Chain: M
Length: 48 nucleotides
Theoretical weight: 15.54 KDa
RNA (30-MER) Chain: N
Molecule details ›
Chain: N
Length: 50 nucleotides
Theoretical weight: 16 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-0454
Expression system: Escherichia coli