› 6o19

X-ray diffraction
1.6Å resolution

Crystal Structure of Pho7 complex with pho1 promoter site 2

Released:
DOI: 10.2210/pdb6o19/pdb
PDB entry 6o19 coloured by chain, front view.
PDB entry 6o19 coloured by chain, side view.
PDB entry 6o19 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Structure of Fission Yeast Transcription Factor Pho7 Bound to pho1 Promoter DNA and Effect of Pho7 Mutations on DNA Binding and Phosphate Homeostasis.
Garg A, Goldgur Y, Sanchez AM, Schwer B, Shuman S
Mol Cell Biol 39 (2019)
PMID: 31010807

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120361 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Uncharacterized transcriptional regulatory protein C27B12.11c Chain: A
Molecule details ›
Chain: A
Length: 59 amino acids
Theoretical weight: 6.76 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O13658 (Residues: 279-336; Coverage: 8%)
Gene names: SPBC27B12.11c, pi067
DNA (5'-D(*TP*TP*AP*TP*TP*CP*GP*GP*AP*AP*AP*TP*TP*AP*AP*AP*AP*AP*CP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.15 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Not provided
DNA (5'-D(*GP*TP*TP*TP*TP*TP*AP*AP*TP*TP*TP*CP*CP*GP*AP*AP*TP*AP*AP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.11 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 53.169Å b: 37.581Å c: 57.564Å
α: 90° β: 104.23° γ: 90°
R-values:
R R work R free
0.182 0.181 0.203
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Transcriptional interference at tandem lncRNA and protein-coding genes: an emerging theme in regulation of cellular nutrient homeostasis.
Shuman S. (2020)
2 more

1 mention without citation

Structure of Fission Yeast Transcription Factor Pho7 Bound to pho1 Promoter DNA and Effect of Pho7 Mutations on DNA Binding and Phosphate Homeostasis.
Garg et al. (2019)