X-ray diffraction
2.4Å resolution

Structural Analysis of a Nitrogenase Iron Protein from Methanosarcina acetivorans: Implications for CO2 Capture by a Surface-Exposed [Fe4S4] Cluster


Function and Biology Details

Reaction catalysed:
8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Nitrogenase iron protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 273 amino acids
Theoretical weight: 29.27 KDa
Source organism: Methanosarcina acetivorans C2A
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q8TJ93 (Residues: 1-273; Coverage: 100%)
Gene names: MA_3895, nifH
Sequence domains: 4Fe-4S iron sulfur cluster binding proteins, NifH/frxC family

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P6522
Unit cell:
a: 96.169Å b: 96.169Å c: 320.244Å
α: 90° β: 90° γ: 120°
R R work R free
0.188 0.186 0.216
Expression system: Escherichia coli BL21(DE3)