› 6ntp

X-ray diffraction
1.89Å resolution

PTP1B Domain of PTP1B-LOV2 Chimera

Released:
DOI: 10.2210/pdb6ntp/pdb
PDB entry 6ntp coloured by chain, front view.
PDB entry 6ntp coloured by chain, side view.
PDB entry 6ntp coloured by chain, top view.
Source organisms:
Primary publication:
Minimally disruptive optical control of protein tyrosine phosphatase 1B.
Hongdusit A, Zwart PH, Sankaran B, Fox JM
Nat Commun 11 788 (2020)
PMID: 32034150

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129258 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 1; non-specific serine/threonine protein kinase Chain: A
Molecule details ›
Chain: A
Length: 448 amino acids
Theoretical weight: 52.15 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P18031 (Residues: 1-298; Coverage: 69%)
  • Canonical: O49003 (Residues: 406-546; Coverage: 15%)
Gene names: NPH1-1, PTP1B, PTPN1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P3121
Unit cell:
a: 89.254Å b: 89.254Å c: 105.747Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.179 0.212
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Engineering an Allosteric Control of Protein Function.
Vishweshwaraiah et al. (2021)
6 more

2 mentions without citation

Minimally disruptive optical control of protein tyrosine phosphatase 1B.
Hongdusit et al. (2020)
1 more