6nqr

X-ray diffraction
2.9Å resolution

Crystal structure of fast switching M159T mutant of fluorescent protein Dronpa (Dronpa2)- Y63(3-NO2Y)

Released:
Source organism: Echinophyllia sp. SC22
Primary publication:
Electrostatic control of photoisomerization pathways in proteins.
Science 367 76-79 (2020)
PMID: 31896714

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 255 amino acids
Theoretical weight: 29.01 KDa
Source organism: Echinophyllia sp. SC22
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5TLG6 (Residues: 3-223; Coverage: 98%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P1
Unit cell:
a: 71.61Å b: 79.43Å c: 85.93Å
α: 89.96° β: 92.53° γ: 94.76°
R-values:
R R work R free
0.22 0.216 0.283
Expression system: Escherichia coli BL21(DE3)