X-ray diffraction
2.25Å resolution

Crystal structure of FBF-2 repeat 5 mutant (C363S, R364Y, Q367S) in complex with 8-nt RNA


Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Fem-3 mRNA-binding factor 2 Chain: A
Molecule details ›
Chain: A
Length: 413 amino acids
Theoretical weight: 46.97 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
  • Canonical: Q09312 (Residues: 164-575; Coverage: 65%)
Gene names: F21H12.5, fbf-2
Sequence domains: Pumilio-family RNA binding repeat
Structure domains: Leucine-rich Repeat Variant
RNA (5'-R(*UP*GP*UP*AP*AP*AP*UP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.54 KDa
Source organism: Caenorhabditis elegans
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P61
Unit cell:
a: 96.397Å b: 96.397Å c: 99.853Å
α: 90° β: 90° γ: 120°
R R work R free
0.162 0.159 0.206
Expression systems:
  • Escherichia coli
  • Not provided